Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil

The Antarctic region is facing a higher risk of hydrocarbon pollution due to increased human activities. Compounds such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic compounds available in fuel are highly stable can reside the environment for prolonged periods if left untreated. isolation native strains needed develop bioremediation applications suitable Antarctica. Strain BS19 was isolated compound carbazole-degrading bacterium from soil through culture enrichment. 16S rRNA gene sequences identified strain member Sphingonium genus. could remove 75% carbazole after 15 days at °C. Whole genome sequencing resulted incomplete genomes 4.77 Mb 96 contigs with lowest GC content among Sphingobium sp. strains. analyses revealed car cluster ant genes cat required complete metabolism source carbon energy. comparison showed similarity Novosphingobium KA1. expression confirmed an RT-PCR analysis indicating involvement predicted degradation. findings this study provide more insight into developing approaches Antarctica other cold environments.